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乙肝病毒在肝癌发生中的确切作用尚不清楚。最近的病毒调节基因HB×的鉴定提示可能直接参与肝癌的发生,乙肝病毒基因具有转录激活物作用的HBx蛋白可能改变宿主基因表达,并导致肝细胞癌的产生。我们通过将完整的HB×基因连同它的调节因子直接导入小鼠的生殖系统,验证了这种可能性。带有这种病毒基因的转基因动物死于进行性的肝病理文化,开始时出现各个病灶,其中肝细胞有改变,随后出现良性腺瘤,进而发生恶性肝癌。雄鼠发生疾病和死亡比雌鼠早。此转基因动物模型用来说明病毒HB_x基因表达后的分子活动经过是较为理想的,这些分子活动结果发生鼠肝癌。
The exact role of hepatitis B virus in the development of liver cancer remains unclear. The recent identification of the viral regulatory gene HB× suggests that it may be directly involved in the development of liver cancer. Hepatitis B virus gene with transcriptional activator HBx protein may change the host gene expression and lead to hepatocellular carcinoma. We verified this possibility by direct introduction of the complete HBx gene along with its regulators into the mouse’s reproductive system. Transgenic animals with this viral gene die from a progressive liver pathology culture, and at the beginning, there are various lesions, of which liver cells have changed, followed by benign adenomas, and then malignant liver cancer. Male mice develop disease and die earlier than female mice. This transgenic animal model was used to demonstrate that the molecular activity of the virus after HB_x gene expression is ideal. These molecular activities result in rat liver cancer.