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结核分枝杆菌 Mycobacterium tuberculsis（M.t）4种耐药基因的研究,了解耐药基因突变情况和耐药水平的关系。108例临床痰标本临床分离株均做传统梯度药敏试验和聚合酶链反应多态-单链构象多态性（PCR-SSCP）试验。结果表明耐SM（rpsL）REP（rpoB）INH（katG）EMB（embB）基因突变率分别为78.5％,68.2％,70.5％,48.6％。其中,上述高耐药株基因突变率分别为86.5％,89.3％,84.3％,35.3％。低耐药株分别为28.5％,16.5％,7.1％。EMB在低耐药区无基因突变。M.t的4种耐药基因联合检测的分析,在国内外很少报道。部分M.t的耐药由耐药基因突变所致,M.t耐药基因突变与耐药水平密切相关,且M.t基因突变绝大多数发生在高耐药区中,也有少部分在低耐区株中发生。
Mycobacterium tuberculsis Mycobacterium tuberculosis (M.t) four kinds of resistance genes to understand the relationship between drug resistance mutations and drug resistance levels. The clinical isolates from 108 clinical sputum samples were subjected to traditional gradient susceptibility testing and polymerase chain reaction polymorphism-single strand conformation polymorphism (PCR-SSCP). The results showed that the mutation rates of embB gene were 78.5%, 68.2%, 70.5% and 48.6%, respectively, in the rpsL REP (rpoB) INH (katG) EMB. Among them, the mutation rates of the above highly resistant strains were 86.5%, 89.3%, 84.3% and 35.3%, respectively. Low-resistant strains were 28.5%, 16.5%, 7.1%. EMB gene mutation in low resistance area. M.t four kinds of resistance genes combined detection of the analysis, rarely reported at home and abroad. The resistance of some Mt was caused by the mutation of resistance gene, and the mutation of Mt resistance gene was closely related to the resistance level. Most of Mt gene mutation occurred in high resistant area and a few of them occurred in low resistant area .