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目的:探讨Skp2、p27、p63和COX-2在宫颈鳞状细胞癌及癌前病变中的表达及意义。方法:采用组织微阵技术和SP免疫组化染色方法。结果:标本77例,其中正常宫颈标本14例,CINI-CINⅡ11例,CINⅢ12例,宫颈鳞状细胞癌40例,其中宫颈癌按细胞分化程度分为:I级8例,Ⅱ级23例,Ⅲ级9例。CINI-CINⅡ,CINⅢ,宫颈鳞状细胞癌三组比较,Skp2表达随着宫颈病变的发展而增强,有显著性差异(Hc=26.664,P<0.05),p27表达随着宫颈病变的发展而减弱,有显著性差异(Hc=15.731,P<0.05)。p63表达随着宫颈病变的发展而增强,有显著性差异(Hc=33.935,P<0.05),COX-2表达随着宫颈病变的发展而增强,有显著性差异(Hc=32.408,P<0.05)。Skp2、p63和COX-2在细胞各分化类型(Ⅰ级,Ⅱ级,Ⅲ级)中的表达无显著性差异(P>0.05)。p27均为阳性表达。结论:Skp2和p27为细胞周期的正、负调控因子,其调控的失调,对宫颈癌形成有促进作用。p63表达于具有高度潜能的基底细胞,从CIN发展至癌,p63的表达显著增强,p63阳性表达与p27的阴性表达是否可作为宫颈鳞状细胞癌诊断的指标有待于进一步证实。
Objective: To investigate the expression and significance of Skp2, p27, p63 and COX-2 in cervical squamous cell carcinoma and precancerous lesions. Methods: Tissue microarray and SP immunohistochemical staining methods were used. Results: There were 77 specimens of normal cervix, including 11 cases of CINI-CINⅡ, 12 cases of CINⅢ and 40 cases of cervical squamous cell carcinoma. Among them, 8 cases were grade I, 23 cases were grade Ⅱ, Grade 9 cases. CINI-CINⅡ, CINⅢ, cervical squamous cell carcinoma in three groups, Skp2 expression increased with the development of cervical lesions, there was a significant difference (Hc = 26.664, P <0.05), p27 expression decreased with the development of cervical lesions , There was a significant difference (Hc = 15.731, P <0.05). The expression of p63 increased with the development of cervical lesions, with significant difference (Hc = 33.935, P <0.05). The expression of COX-2 increased with the development of cervical lesions (Hc = 32.408, P <0.05 ). There was no significant difference in the expression of Skp2, p63 and COX-2 in all cell types (Ⅰ, Ⅱ and Ⅲ) (P> 0.05). p27 are positive expression. Conclusion: Skp2 and p27 are positive and negative regulators of cell cycle. The regulation of Skp2 and p27 may promote the formation of cervical cancer. p63 is expressed in basal cells with high potential. The expression of p63 is significantly increased from CIN to carcinoma. Whether p63 positive expression and p27 negative expression can be used as an indicator of cervical squamous cell carcinoma remains to be confirmed.