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目的:探讨Src激酶特异性抑制剂PP2对人胆管癌QBC939细胞侵袭能力的影响和机制。方法:通过Western Blotting技术检测PP2对人胆管癌QBC939细胞中Src激酶活化的影响;用Transwell小室法观察PP2对QBC939细胞的影响;用RT-PCR和Western Blotting技术检测PP2对QBC939细胞侵袭能力相关分子的作用。结果:实验组p-Src蛋白表达水平明显低于对照组,差异具有统计学意义(P<0.05);实验组QBC939细胞体外侵袭能力较对照组显著降低,差异具有统计学意义(P<0.05);与对照组相比,实验组E-cadherin表达显著增强,CD44表达明显减弱,差异具有统计学意义(P<0.05)。结论:PP2通过抑制Src激酶活化,增强E-cadherin表达、减弱CD44表达,抑制人胆管癌QBC939细胞侵袭能力。
Objective: To investigate the effect and mechanism of Src kinase-specific inhibitor PP2 on invasiveness of human cholangiocarcinoma QBC939 cells. Methods: The effect of PP2 on the activation of Src kinase in human cholangiocarcinoma QBC939 cells was detected by Western Blotting. The effect of PP2 on QBC939 cells was observed by Transwell chamber assay. The effect of PP2 on the invasion ability of QBC939 cells was detected by RT-PCR and Western Blotting. Role. Results: The expression of p-Src protein in experimental group was significantly lower than that in control group (P <0.05). The in vitro invasive ability of QBC939 cells in experimental group was significantly lower than that of control group (P <0.05) ; Compared with the control group, the expression of E-cadherin in experimental group was significantly increased, while the expression of CD44 was significantly decreased. The difference was statistically significant (P <0.05). Conclusion: PP2 can inhibit the invasion of human cholangiocarcinoma QBC939 cells by inhibiting the activation of Src kinase, increasing the expression of E-cadherin, decreasing the expression of CD44.