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【目的】揭示以亚硝氮为唯一氮源生长的海洋紫色硫细菌去除水体中无机三态氮的特征和规律。【方法】在光照厌氧环境下,以乙酸盐为唯一有机物,在分别以氨氮、亚硝态氮、硝态氮为唯一氮源和三氮共存的模拟水体中,采用Nessler’s试剂分光光度法、N-(1-萘基)-乙二胺分光光度法和紫外分光光度法分别测定水体中氨氮、亚硝态氮和硝态氮的含量,比浊法测定菌体生物量。【结果】随着时间的延长,海洋紫色硫细菌Marichromatium gracile YL28分别在氨氮、亚硝态氮和硝态氮为唯一氮源的水体中对三氮的去除量增加,生物量增大,水体pH升高,并逐渐趋于平衡;YL28对氨氮的最大去除量和最大耐受浓度分别为9.64 mmol/L和36.64 mmol/L,当氨氮浓度低于3.21 mmol/L时,去除率可达97.61%以上;与氨氮相比,以亚硝态氮和硝态氮为唯一氮源,菌体的生长速率、生物量和水体最终pH较低,但对亚硝态氮和硝态氮的去除速率和去除量仍然很高,当亚硝态氮和硝态氮浓度分别达13.50 mmol/L和22.90 mmol/L时,YL28仍能够完全去除。在三氮共存的水体中,YL28也能良好的去除无机三态氮,对亚硝态氮和硝态氮去除能力更强。【结论】在模拟水体中,海洋紫色硫细菌YL28能够分别以氨氮、亚硝态氮和硝态氮为唯一氮源生长,具有良好的耐受和去除无机三态氮的能力,尤其对亚硝态氮具有良好的去除能力。本研究为进一步开发高效脱氮,尤其是去除亚硝态氮的不产氧光合细菌水质调节剂奠定了基础,也为微生物制剂的合理应用提供参考。
【Objective】 The objective of this study was to reveal the characteristics and laws of inorganic ternary nitrogen removal from marine purple sulfur bacteria with nitrite nitrogen as the only nitrogen source. 【Method】 Acetate was the only organic matter in simulated sunlight and anaerobic conditions. Nessler’s reagent spectrophotometry was used to simulate the coexistence of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen as the only nitrogen source and triazine nitrogen. , N- (1-naphthyl) -ethylenediamine spectrophotometry and ultraviolet spectrophotometry were used to determine the contents of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen respectively. The turbidimetric method was used to determine the microbial biomass. 【Result】 With the extension of time, the removal rate of triazitium increased and the biomass of marine purple sulfur bacteria Marichromatium gracile YL28 increased respectively in the water bodies with ammonia nitrogen, nitrite nitrogen and nitrate nitrogen as the only nitrogen sources. The water pH And the maximum tolerated concentration of YL28 was 9.64 mmol / L and 36.64 mmol / L, respectively. When ammonia concentration was lower than 3.21 mmol / L, the removal rate of YL28 reached 97.61% Compared with ammonia nitrogen, nitrite and nitrate were the only nitrogen sources. The growth rate, biomass and final pH of the bacteria were lower, but the removal rate of nitrite and nitrate and The removal rate is still very high. When the concentrations of nitrite and nitrate were 13.50 mmol / L and 22.90 mmol / L respectively, YL28 could still be completely removed. YL28 is also able to remove inorganic trinitrogen well in the coexisting water of triammonium, and has a stronger ability to remove nitrite and nitrate nitrogen. 【Conclusion】 In simulated water, marine purple sulfur bacteria YL28 can grow ammonia nitrogen, nitrite nitrogen and nitrate nitrogen as the only nitrogen sources respectively, which has good tolerance and ability to remove inorganic tristate nitrogen, especially for nitrite Nitrogen has a good removal ability. This study lays the foundation for the further development of high efficiency nitrogen removal, especially non-oxygen-producing photosynthetic bacteria water quality regulator for removing nitrite nitrogen, and also provides a reference for the rational application of microbial agents.