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目的:探讨甲状腺素（Tn 4）及丙硫氧嘧啶（PTU）对人脐静脉内皮细胞（HUVEC细胞）一氧化氮合酶表达的影响。n 方法:体外培养HUVEC细胞，实验分为6组，分别为对照组（未加Tn 4及PTU），10n -9、10n -7、10n -4 mol/L Tn 4组，PTU组（5 μg/ml PTU），10n -4 mol/L Tn 4 + PTU组，作用时间为24 h。采用CCK-8法检测细胞活性；硝酸还原酶法检测细胞培养液一氧化氮（NO）、总一氧化氮合酶（TNOS）、内皮型一氧化氮合酶（eNOS）含量；蛋白质印迹法检测eNOS蛋白表达水平。n 结果:6组细胞存活率[（100.00 ± 0.00）%、（96.73 ± 1.17）%、（86.20 ± 7.54）%、（47.37 ± 9.10）%、（53.37 ± 5.47）%、（53.40 ± 8.84）%]比较差异有统计学意义（n F = 29.42，n P < 0.05）；与对照组比较，10 n -7、10n -4 mol/L Tn 4组，PTU组和10n -4 mol/L Tn 4 + PTU组细胞存活率显著降低（n P均< 0.05）。6组细胞培养液NO、TNOS含量比较差异有统计学意义（n F = 3.93、3.46，n P均< 0.05）；与对照组比较，PTU组TNOS含量显著降低（n P < 0.05）；与10 n -4 mol/L Tn 4组比较，PTU、10n -4 mol/L Tn 4 + PTU组NO含量显著降低（n P均 0.05）。n 结论:高浓度Tn 4可造成体外培养HUVEC细胞活性的损伤，PTU通过调节NO、TNOS起到对其的缓解作用，具体作用机制还需进一步分子生物实验的深入研究。n “,”Objective:To investigate the effects of thyroxine (Tn 4) and propylthiouracil (PTU) on nitric oxide synthase expression in human umbilical vein endothelial cells (HUVEC cells).n Methods:HUVEC cells were cultured n in vitro, the experiment was divided into 6 groups: control (without Tn 4 and PTU), 10n -9, 10n -7, 10n -4 mol/L Tn 4, PTU (5 μg/ml PTU), and 10 n -4 mol/L Tn 4 + PTU, the action time was 24 h. CCK-8 method was used to detect cell activity; nitrate reductase method was used to detect nitric oxide (NO), total nitric oxide synthase (TNOS) and endothelial nitric oxide synthase (eNOS) contents; Western blotting was used to detect eNOS protein expression level.n Results:The differences of cell survival rates [(100.00 ± 0.00)%, (96.73 ± 1.17)%, (86.20 ± 7.54)%, (47.37 ± 9.10)%, (53.37 ± 5.47)%, (53.40 ± 8.84)%] among the 6 groups were statistically significant (n F = 29.42, n P < 0.05). Compared with the control group, the cell survival rates of 10 n -7, 10n -4 mol/L Tn 4, PTU, and 10n -4 mol/L Tn 4 + PTU groups were significantly reduced (n P < 0.05). There were statistically significant differences in the contents of NO and TNOS among the 6 groups ( n F = 3.93, 3.46, n P < 0.05). Compared with the control group, the TNOS content in PTU group was significantly reduced ( n P < 0.05); compared with the 10 n -4 mol/L Tn 4 group, the NO contents in PTU and 10n -4 mol/L Tn 4 + PTU groups were significantly reduced (n P 0.05).n Conclusions:High concentration of Tn 4 can cause damage to the activity of HUVEC cells cultured n in vitro, and PTU can alleviate it by regulating NO and TNOS. The specific mechanism of action still needs to be further studied in molecular biological experiments.n