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目的探讨宫颈癌组织中人乳头状瘤病毒(HPV)16E6mRNA表达与survivin蛋白表达的相关性。方法采用半定量PCR技术和免疫组化链霉菌抗生物素蛋白-过氧化物酶连接法,检测慢性宫颈炎、宫颈上皮内瘤变(CIN)及宫颈癌共148例患者宫颈组织中HPV16E6mRNA及survivin蛋白的表达。结果148例患者中,HPV16阳性共37例,其中慢性宫颈炎5例、CINⅠ6例、CINⅡ～Ⅲ11例及宫颈癌15例。慢性宫颈炎、CINⅠ、CINⅡ～Ⅲ及宫颈癌组织中,HPV16E6mRNA的表达水平分别为0.3±0.4、0.6±0.4、1.8±0.6及2.4±0.6;survivin蛋白阳性表达率分别为7%、31%、63%及84%。CINⅡ～Ⅲ及宫颈癌组织中,HPV16E6mRNA的表达水平及survivin蛋白阳性表达率均显著高于慢性宫颈炎及CINⅠ组织,两者比较,差异均有统计学意义(P<0.01)。宫颈癌组织中,HPV16E6mRNA的表达水平与survivin蛋白阳性表达率呈显著正相关关系(γs=0.62,P<0.05)。结论HPV16E6mRNA的表达水平与宫颈病变的进展有关,survivin蛋白阳性表达率升高可能与宫颈癌的发生、发展密切相关。
Objective To investigate the correlation between HPV16E6 mRNA expression and survivin protein expression in cervical cancer. Methods The expression of HPV16E6 mRNA and survivin in cervical tissue of 148 patients with chronic cervicitis, cervical intraepithelial neoplasia (CIN) and cervical cancer were detected by semi-quantitative PCR and immunohistochemical streptavidin-peroxidase ligation Protein expression. Results Of the 148 patients, HPV16 was positive in 37 cases, including 5 cases of chronic cervicitis, 6 cases of CINⅠ, 11 cases of CINⅡ ~ Ⅲ and 15 cases of cervical cancer. The expression of HPV16E6 mRNA in chronic cervicitis, CINⅠ, CINⅡ ~ Ⅲ and cervical cancer tissues were 0.3 ± 0.4,0.6 ± 0.4,1.8 ± 0.6 and 2.4 ± 0.6, respectively. The positive rate of survivin protein expression was 7%, 31% 63% and 84%. The expression of HPV16E6 mRNA and the positive rate of survivin protein in CINⅡ ~ Ⅲ and cervical cancer tissues were significantly higher than those in chronic cervicitis and CINⅠ tissues, the difference was statistically significant (P <0.01). In cervical cancer, the expression of HPV16E6 mRNA was positively correlated with the positive rate of survivin protein (γs = 0.62, P <0.05). Conclusion The expression of HPV16E6 mRNA is related to the progression of cervical lesions. The increased expression of survivin protein may be closely related to the occurrence and development of cervical cancer.