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目的:确定芫花药材致肝毒性部位,为全面反映并控制其毒性部位的质量建立该部位超高效液相色谱指纹图谱。方法:通过血清生化指标和组织病理学筛选芫花致肝毒性部位;并以采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×50mm,1.7μm),乙腈-0.05%磷酸溶液为流动相,梯度洗脱,210 nm为检测波长建立该部位UPLC指纹图谱。结果:确定了芫花致肝毒性部位为其乙醇提取物的氯仿萃取物;并建立了该部位的UPLC指纹图谱共有模式,标定了17个共有峰,指认了8个色谱峰,14批芫花药材致肝毒性部位的相似度为0.890～0.999。结论:芫花致肝毒性部位为其乙醇提取物的氯仿萃取物,所建立的UPLC指纹图谱,可为芫花临床安全用药提供依据。
OBJECTIVE: To determine the hepatotoxicity caused by Daphne genkwa, and to establish the HPLC fingerprint of this site in order to fully reflect and control the quality of its toxic site. Methods: The hepatotoxic sites of Daphne genkwa were screened by serum biochemical indexes and histopathology. The mobile phase was eluted with ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) and acetonitrile-0.05% phosphoric acid solution with gradient elution , 210 nm for the detection wavelength to establish the site UPLC fingerprinting. Results: The chloroform extract of the ethanol extract from the liver of Daphne genkwa was identified. The common UPLC fingerprinting pattern of the site was established, 17 common peaks were calibrated, 8 peaks were identified, 14 batches of Daphne genkwa The similarity of the drug-induced liver toxicity site is 0.890 ~ 0.999. CONCLUSION: The hepatotoxic region of Daphne genkwa is the chloroform extract of its ethanol extract. The established UPLC fingerprinting can provide basis for clinical safety of Daphne genkwa.