在 0 .0 5mol/L ,pH =6 .0的六次甲基四胺溶液中 ,室温条件下显色剂 2 - (2 ,3,5 -三氮唑偶氮 ) - 5 -甲氨基苯甲酸 (TZADMAB以下简称TZ)与Pd(Ⅱ )形成兰色配合物 ,其组成Pd(Ⅱ )∶TZ =1∶1,最大吸收波长位于 6 0 8nm处。在此条件下 ,TZ -Pd(Ⅱ )配合物与鱼精子DNA能迅速化合生成超分子化合物 ,使化合物在一定范围内的吸光度不断增大。吸光度与DNA的质量浓度在 0 .0 0 1- 0 .0 1mg .mL- 1范围内呈线性关系 ,线性方程为ΔA =- 0 .0 4 37+ 0 .0 0 73c(× 10 - 6 g/L) ,相关系数为r =0 .995 4,检测限为 1μg/mL。因此 ,这一方法可以用来进行DNA浓度的测定。 The color reagent 2 - (2, 3,5 - triazolylazo) - 5 - methylaminobenzene was reacted with hexamethylenetetramine at 0. 5mol / L and pH = Formic acid (TZADMAB, hereinafter referred to as TZ) forms a blue complex with Pd (Ⅱ), and its composition Pd (Ⅱ): TZ = 1: 1 and its maximum absorption wavelength is at 680 nm. Under these conditions, the TZ-Pd (II) complex and fish sperm DNA can rapidly combine to form a supramolecular compound, which increases the absorbance of the compound within a certain range. The absorbance and the mass concentration of DNA showed a linear relationship in the range of 0.001-0.01 mg · mL-1. The linear equation was ΔA = -0.043.7 +0.073c (× 10-6 g / L), the correlation coefficient is r = 0 .995 4, the detection limit is 1μg / mL. Therefore, this method can be used to determine the concentration of DNA.