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以红霉素为模板分子,采用溶胶-凝胶印迹技术,合成对红霉素具有特异选择识别性能的印迹聚合材料.采用红外光谱、扫描电子显微镜、热重分析以及比表面积测定技术对印迹聚合物的结构和表面性能进行详细探讨,结果表明所得印迹聚合物比表面积为266.2m2/g,粒径为600nm左右的颗粒材料.选择3种抗生素类药物进行选择吸附研究,结果表明,该聚合物对红霉素的分离因子最高达到2.20.优化固相萃取条件,结果表明用甲醇:乙酸(75:25,V/V)作为洗脱剂时对红霉素的洗脱效果最好.结合液相色谱技术,该印迹材料成功的分离和富集红霉素肠溶片中的红霉素,回收率达到104.5%。
Erythromycin was used as a template molecule to synthesize imprinted polymeric materials with selective recognition of erythromycin by sol-gel technique.METHODS were characterized by FTIR, SEM, TGA and surface area measurement The structure and surface properties of the polymers were investigated in detail, and the results showed that the obtained imprinted polymer had a specific surface area of 266.2m2 / g and a particle size of about 600nm. Three kinds of antibiotics were selected for selective adsorption. The results showed that the polymer The separation factor of erythromycin reached up to 2.20.The conditions of solid-phase extraction were optimized.The results showed that the elution of erythromycin was the best when methanol: acetic acid (75:25, V / V) Phase chromatography technology, the imprinting material successfully isolated and enriched erythromycin in erythromycin enteric-coated tablets, the recovery rate reached 104.5%.