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目的:体外模拟高糖病理微环境,探讨黄芪多糖对小鼠肾小球足细胞Nephrin表达的影响。方法:试验一:体外培养小鼠肾小球足细胞,分别为D-葡萄糖30mmol/L+黄芪多糖干预组(0.0g/L、0.1g/L、0.2g/L、0.4g/L和0.8g/L);干预时间为72h。试验二:分别为D-葡萄糖30mmol/L+黄芪多糖干预(浓度为0.X g/L),干预时间分别为0h、3h、6h、12h、24h、48h和72h。分别采用流式细胞计数检测各组足细胞Nephrin表达。结果:随着黄芪多糖干预浓度的递增,足细胞表面Nephrin表达逐渐上调,呈浓度依赖性(P<0.05);随着黄芪多糖干预时间的延长,足细胞表面Nephrin表达逐渐上调,呈时间依赖性。结论:在高糖刺激下,黄芪多糖干预可上调肾小球足细胞Nephrin表达,是黄芪治疗糖尿病肾病蛋白尿的可能作用靶点。
OBJECTIVE: To simulate the pathological microenvironment of high glucose in vitro and investigate the effect of astragalus polysaccharide on nephrin expression in mouse glomerular podocytes. Methods: Test 1: Mouse glomerular podocytes were cultured in vitro and were D-glucose 30 mmol/L + Astragalus polysaccharide intervention groups (0.0 g/L, 0.1 g/L, 0.2 g/L, 0.4 g/L, and 0.8 g, respectively). /L); Intervention time is 72h. The second experiment: D-glucose 30mmol/L + astragalus polysaccharide intervention (concentration 0.Xg/L), intervention time 0h, 3h, 6h, 12h, 24h, 48h and 72h, respectively. Flow cytometry was used to detect Nephrin expression in podocytes of each group. RESULTS: With the increasing intervention concentration of Astragalus polysaccharides, the expression of Nephrin on the surface of podocytes was gradually increased in a concentration-dependent manner (P<0.05). With the prolonged intervention time of APS, the expression of Nephrin on the surface of podocytes was gradually increased, which was time-dependent. . Conclusion: Under high glucose stimulation, astragalus polysaccharide intervention can upregulate nephrin expression in glomerular podocytes, which is a possible target for astragalus to treat diabetic nephropathy proteinuria.