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在世界范围内,癌症的死亡率仍在逐年上升.循环肿瘤细胞(circulating tumor cells,CTCs)是指从原发肿瘤脱落并进入血液循环系统的细胞,可能引发肿瘤转移并入侵其他正常组织和器官.因此,CTCs的检测结果可以作为癌症病人疗效和预后的评价指标.但是CTCs的数量及其稀少,使得CTCs的检测尤为困难.在癌症转移的病人中,每毫升血液约含有10～100个CTCs.利用经生物活性材料表面修饰的微流控器件,可以从血液中分离出CTCs.这是一项跨学科的挑战,需要来自不同学科背景的专家们共同参与,如细胞生物学、表面化学、流体力学及微纳加工技术等.该文首先介绍了CTCs的细胞生物学基础,然后总结了当前分离CTCs的主要微流控技术,包括基于细胞-配体作用、磁力作用和过滤等,最后综述了基于微流控技术的CTC检测和计数、在体CTC成像等最新研究进展.
Worldwide, cancer death rates are still increasing year by year.Circulating tumor cells (CTCs) refer to cells that fall from the primary tumor and enter the blood circulation system, which may trigger tumor metastasis and invade other normal tissues and organs Therefore, CTCs test results can be used as an indicator of efficacy and prognosis of cancer patients, but the number of CTCs and its sparse, making the detection of CTCs is particularly difficult in patients with cancer metastasis, per ml of blood contains about 10 to 100 CTCs CTCs can be separated from blood using microfluidic devices that are surface-modified with biologically active materials.This is an interdisciplinary challenge that requires the participation of experts from different disciplines, such as cell biology, surface chemistry, Fluid mechanics and micro / nanofabrication technology, etc. This paper firstly introduces the cellular biology of CTCs and then summarizes the current microfluidic technologies for CTCs isolation, including cell-ligand based, magnetism and filtration, etc. Based on the microfluidic CTC detection and counting, in vivo CTC imaging and other recent research progress.