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将联苯胺、邻联甲苯胺、苯胺、邻甲苯胺四种芳香胺类化合物,分别经口给予雄性SD大鼠,收集实验后24小时内尿液,调节尿pH至7,用乙醚抽提、蒸干,残留物以二甲亚砜溶解,选TA98、TA100菌株,进行鼠伤寒沙门氏菌/微粒体酶致突变试验。S9用多氯联苯诱导的雄性SD大鼠制备。同时与上述四种芳香胺化合物体外检测致突变活性结果比较。实验表明联苯胺、邻联甲苯胺组鼠尿提取物,经活化致突变作用较体外检测明显增强。苯胺、邻甲苯胺于体外皆无致突变性,而经体内转化实验鼠尿皆具致突变性。高压液相色谱与IR光谱分析,证明联苯胺实验性鼠尿提取物中,存在N-乙酰联苯胺。后者于体外测试
The four benzidine, o-tolidine, aniline, o-toluidine four aromatic amine compounds were orally administered to male SD rats, urine collected within 24 hours after the experiment to adjust the urine pH to 7, extracted with ether, Evaporated to dryness and the residue was dissolved in dimethyl sulfoxide. TA98 and TA100 strains were selected for mutagenicity test of Salmonella typhimurium / microsome. S9 Prepared with polychlorinated biphenyl induced male SD rats. At the same time with the above four aromatic amine compounds in vitro mutagenic activity results were compared. Experiments show that the benzidine, o-tolidine group of rats urine extract, the role of activation caused by mutagenesis was significantly enhanced than in vitro testing. Aniline, o-toluidine in vitro are not mutagenic, and in vivo transformation of experimental mice are all mutagenic. High-pressure liquid chromatography and IR spectroscopy showed that benzidine was present in the benzidine-induced rat urine extract. The latter is tested in vitro