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LNG(LONGIFOLIA)基因在模式植物叶片和果实生长发育过程中发挥重要的调控作用。然而,茄科作物中的LNG基因功能尚不明确。本研究运用同源克隆的方法从“Micro-Tom”番茄矮化品种中鉴定到两个LNG基因,基于其与拟南芥AtLNG1和AtLNG2的序列相似性,命名为SlLNG1和SlLNG2。并对这两个家族成员进行了基因结构、蛋白特征、序列保守结构域和进化关系等分析。结果表明:SlLNG1和SlLNG2分别位于第2染色体和第3染色体,编码产物与马铃薯同源蛋白的相似性最高。荧光定量PCR实验结果发现,SlLNG1和SlLNG2基因在番茄的各组织中的表达具有较大差异,表现出明显的组织特异性。SlLNG1在花中的表达量最高,而SlLNG2在叶片中的表达最高。此外,在SlLNG1和SlLNG2的1.5 kb启动子区域发现多个光响应、胁迫响应和水杨酸响应的顺式作用元件。本研究为深入研究番茄LNG基因的结构、功能和调控机制奠定了基础。
The LONGIFOLIA gene plays an important regulatory role in the growth and development of model plant leaves and fruits. However, the function of LNG genes in solanaceous crops is not clear. In this study, two LNG genes were identified from homologous clones from “Micro-Tom ” tomato dwarfing varieties and named as SlLNG1 and SlLNG2 based on their sequence similarity with Arabidopsis AtLNG1 and AtLNG2. The two family members were analyzed gene structure, protein features, conserved sequence domains and evolutionary relationships. The results showed that SlLNG1 and SlLNG2 were located on chromosome 2 and 3, respectively, and the similarity of coding products to potato homologues was the highest. Fluorescent quantitative PCR results showed that the expression of SlLNG1 and SlLNG2 genes in the tissues of tomato were quite different and showed obvious tissue specificity. SlLNG1 had the highest expression level in flowers, while SlLNG2 had the highest expression in leaves. In addition, multiple cis-acting elements of photo-response, stress response and salicylic acid were found in the 1.5 kb promoter region of SlLNG1 and SlLNG2. This study laid the foundation for further study on the structure, function and regulatory mechanism of tomato LNG gene.