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目的:本研究旨在调查高温环境下运动时,水或运动饮料补液对血清HSP70水平和DNA损伤的影响。方法:韩国延世大学招募运动年限为6-11年的10名男性大学生运动员参加此项研究。受试者以75%心率储备强度在跑步机上进行4种补液条件下的测试:保持18℃中温环境,不补液(T);32℃高温环境,不补液(H);32℃高温环境下饮水(HW);32℃高温环境下补充运动饮料(HS)。分别在基线状态(PEB)、运动刚结束(IAE)、运动结束60 min(60MPE)3个时间点采集血样。结果:运动时皮肤温度显著升高,且运动刚结束时,H状态皮肤温度显著高于T和HS。在所有条件下,运动刚结束时血清HSP70比运动前显著增长,且H状态较T更高。在所有测试中,运动结束时的淋巴细胞DNA损伤(尾部DNA、尾长、尾矩)较运动前有显著增加。运动结束时,HS相比H可缓解DNA损伤(尾矩)。结论:急性运动可导致血清HSP70升高和淋巴细胞DNA损伤;在高温环境下运动时,运动饮料补液可防止脱水、减少热应激,从而减弱HSP反应与DNA损伤。
OBJECTIVE: This study was designed to investigate the effects of water or sports drink rehydration on serum HSP70 level and DNA damage during exercise at high temperature. Methods: Ten male college athletes who were recruited from Yonsei University in Korea for six to eleven years participated in the study. Subjects underwent 75% heart rate reserve intensity on a treadmill under four rehydration conditions: maintained at 18 ° C in a medium temperature environment without rehydration (T); 32 ° C in a high-temperature environment without rehydration (H); 32 ° C in a high-temperature environment (HW); 32 ° C high temperature environment, add sports drinks (HS). Blood samples were collected at baseline, PEB, IAE, and 60 min (60 MPE) respectively. Results: The skin temperature increased significantly during exercise, and the skin temperature in H state was significantly higher than T and HS immediately after exercise. Under all conditions, serum HSP70 significantly increased at the end of exercise compared with before exercise, and the H state was higher than T. In all tests, lymphocyte DNA damage (tail DNA, tail length, tail moment) at the end of exercise was significantly increased compared to pre-exercise. At the end of exercise, HS compared to H can alleviate DNA damage (tail moment). CONCLUSION: Acute exercise can lead to elevated serum HSP70 and DNA damage of lymphocytes. When exercised in high temperature environment, exercise drink rehydration can prevent dehydration and reduce heat stress, thus attenuating HSP reaction and DNA damage.