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目的研究金乌骨通胶囊对去卵巢大鼠骨质疏松的治疗作用。方法雌性Wistar大鼠40只被随机分为正常对照组、假手术组、卵巢切除组、实验组,每组10只。骨质疏松模型成功后,实验组大鼠灌服金乌骨通胶囊,正常对照组、假手术组和卵巢切除组按照实验组的方法给大鼠灌服等容积的蒸馏水,治疗3个月,测定大鼠腰椎和胫骨上端骨密度及外周血清中雌激素(E2)、降钙素(CT)的含量。结果假手术组腰椎、胫骨上端骨密度明显高于卵巢切除组及实验组(P<0.05),但与正常对照组比较差异未见统计学意义(P>0.05);实验组腰椎、胫骨上端骨密度明显高于卵巢切除组(P<0.01)。假手术组血清E2明显高于卵巢切除组及实验组(P<0.01),但与正常对照组比较差异未见统计学意义(P>0.05);实验组血清E2虽较卵巢切除组有所升高,但差异未见统计学意义(P>0.05)。卵巢切除组血清CT较假手术组明显降低(P<0.01),正常对照组、实验组与假手术组比较差异未见统计学意义(P>0.05);实验组血清CT较卵巢切除组明显升高,差异有统计学意义(P<0.01)。结论金乌骨通胶囊减轻骨吸收、促进骨形成、延缓骨质疏松的发生和发展可能与卵巢切除大鼠血清CT升高有关,而且不依赖于雌激素的表达。
Objective To study the therapeutic effect of Jinwu Gutong Capsule on osteoporosis in ovariectomized rats. Methods Forty female Wistar rats were randomly divided into normal control group, sham operation group, ovariectomized group and experimental group, with 10 rats in each group. After successful osteoporosis model, rats in experimental group were fed with Jinwu Gutong capsule, normal control group, sham operation group and ovariectomized group. The rats were dosed with the same volume of distilled water according to the experimental group for 3 months. The BMD of lumbar vertebrae and tibia and the content of estrogen (E2) and calcitonin (CT) in peripheral serum of rats were measured. Results Compared with the ovariectomized group and the experimental group, the BMD of lumbar spine and tibia in the sham operation group was significantly higher than that in the ovariectomized group and the experimental group (P <0.05), but there was no significant difference between the sham operation group and the normal control group (P> 0.05) The density was significantly higher than ovariectomy group (P <0.01). Serum E2 in sham operation group was significantly higher than that in ovariectomized group and experimental group (P <0.01), but there was no significant difference with normal control group (P> 0.05). Serum E2 in experimental group was higher than that in ovariectomized group High, but the difference was not statistically significant (P> 0.05). Serum CT in ovariectomized group was significantly lower than that in sham-operation group (P <0.01), but there was no significant difference between normal control group and sham operation group (P> 0.05). Serum CT in experimental group was significantly higher than that in ovariectomized group High, the difference was statistically significant (P <0.01). Conclusion Jinwu Gutong Capsule can reduce bone resorption, promote bone formation and delay the occurrence and development of osteoporosis, which may be related to the increase of serum CT in ovariectomized rats, and not depend on the expression of estrogen.