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目的观察亚硒酸钠处理对亚慢性氯化镉中毒大鼠肝、肾结构的影响,为氯化镉慢性中毒的防治和亚硒酸钠蓄积性毒性研究提供依据。方法雄性SD大鼠54只,随机分成3组。对照组大鼠饮用蒸馏水;其余两组大鼠饮用含30 mg/L氯化镉蒸馏水;硒干预组大鼠给予5μmol/kg亚硒酸钠溶液灌胃。结果与对照组比较,氯化镉组大鼠组织中GSH-Px及SOD活性降低、MDA含量增加;硒干预组大鼠组织GSH-Px及SOD活性显著升高、MDA含量降低。组织病理切片观察结果,单纯给氯化镉组大鼠肾小球肿胀或固缩,肾小囊囊腔增大,肾小管上皮细胞变性坏死并脱落等病理损伤。硒干预组大鼠肝、肾脏组织结构未见明显损伤。结论饮用含氯化镉的水源可致肾脏组织明显损伤,低剂量亚硒酸钠可拮抗氯化镉对肾组织的损伤作用,对氯化镉所致组织氧化损伤有一定的修复作用。
Objective To observe the effects of sodium selenite treatment on the structure of liver and kidney in rats exposed to subchronic cadmium chloride (CdCl2), and provide evidence for the prevention and cure of chronic poisoning of cadmium chloride and accumulation of sodium selenite. Methods Fifty-four male SD rats were randomly divided into three groups. Rats in the control group drank distilled water. The remaining two groups of rats were drank with 30 mg / L cadmium chloride distilled water. Rats in the selenium intervention group were administered with 5 μmol / kg sodium selenite solution. Results Compared with the control group, the activities of GSH-Px and SOD in the cadmium chloride group decreased and the MDA content increased. The activities of GSH-Px and SOD in the tissue of the cadmium chloride group increased significantly and MDA content decreased. The results of histopathological observation showed that the cadmium chloride group had glomerular swelling or pyknosis, renal cysts enlarged, renal tubular epithelial cells degenerated, necrotic and exfoliated. Selenium intervention group rats liver and kidney tissue structure showed no significant damage. Conclusions Drinking water containing cadmium chloride can cause obvious damage to the kidney tissue. Low dose sodium selenite can antagonize the damage of cadmium chloride on the renal tissue and repair the tissue oxidative damage induced by cadmium chloride.