本文用计算机辅助单纯形优化高效液相色谱法分离、测定了精炼植物油副产物中维生索E的各种异构体。探讨了单纯形优化法指导高效液相色谱选择分离的实验条件,并编写了实用的计算机程序。实验结果表明,本法可使比较困难的条件选择工作有章可循,从而提高工作效率。 In this paper, computer-assisted simplex-optimized high performance liquid chromatography separation, determination of the refined vegetable oil byproducts of vitamin E various isomers. The experimental conditions for selecting and separating high performance liquid chromatography guided by simplex optimization method were discussed, and a practical computer program was written. The experimental results show that this method can make the selection of the more difficult conditions to be rule-based, so as to improve work efficiency.