mRNA expression of DOK1-6 in human breast cancer

来源 :World Journal of Clinical Oncology | 被引量 : 0次 | 上传用户:yangbao_2002
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
AIM:To examine the expression of downstream of tyrosine kinase(DOK)1-6 genes in normal and breast cancer tissue and correlated this with several clinicopathological and prognostic factors.METHODS:DOK1-6 m RNA extraction and reverse transcription were performed on fresh frozen breast cancer tissue samples(n = 112) and normal background breast tissue(n = 31). Tissues were collected between 1991 and 1996 at two centres and all patients underwent mastectomy and ipsilateral axillary node dissection. All tissues were randomly numbered and the details were only made known after all analyses were completed. Transcript levels of expression were determined using real-time polymerase chain reaction and analyzed against TNM stage, tumour grade and clinical outcome over a 10-year follow-up period.RESULTS:DOK-2 and DOK-6 expression decreased with increasing TNM stage. DOK-6 expression decreased with increasing Nottingham Prognostic Index(NPI) [NPI-1 vs NPI-3(mean copy number 15.4 vs 0.22, 95%CI:2.7-27.6, P = 0.018) and NPI-2 vs NPI-3(mean copy number 7.6 vs 0.22, 95%CI:0.1-14.6, P = 0.048)]. After a median follow up period of 10 years, higherlevels of DOK-2 expression were found among patients who remained disease-free compared to those who developed local or distant recurrence(mean copy number 3.94 vs 0.0000096, 95%CI:1.0-6.85, P = 0.0091), and distant recurrence(mean copy number 3.94 vs 0.0025, 95%CI:1.0-6.84, P = 0.0092). Patients who remained disease-free had higher levels of DOK-6 expression compared to those who died from breast cancer.CONCLUSION:Decreasing expression levels of DOK-2 and DOK-6 with increased breast tumour progression supports the notion that DOK-2 and DOK-6 behave as tumour suppressors in human breast cancer. AIM: To examine the expression of downstream of tyrosine kinase (DOK) 1-6 genes in normal and breast cancer tissue and correlated with several clinicopathological and prognostic factors. METHODS: DOK 1-6 m RNA extraction and reverse transcription were performed on fresh frozen Tissues were collected between 1991 and 1996 at two centers and all patients underwent mastectomy and ipsilateral axillary node dissection. All tissues were randomly numbered and the details were only made known after all analyzes were completed. Transcript levels of expression were determined using real-time polymerase chain reaction and analyzed against TNM stage, tumor grade and clinical outcome over a 10-year follow-up period .RESULTS: DOK-2 and DOK -6 expression decreased with increasing TNM stage. DOK-6 expression decreased with increasing Nottingham Prognostic Index (NPI) [NPI-1 vs NPI-3 (mean copy number 15.4 vs 0.22, 95% CI: 27.6, P = 0.018) and NPI-2 vs NPI-3 (mean copy number 7.6 vs. 0.22, 95% CI: 0.1-14.6, P = 0.048)]. After a median follow up period of 10 years, higher levels of DOK- 2 expression were found among patients who remained disease-free compared to those who developed local or distant recurrence (mean copy number 3.94 vs 0.0000096, 95% CI: 1.0-6.85, P = 0.0091), and distant recurrence (mean copy number 3.94 vs 0.0025, 95% CI: 1.0-6.84, P = 0.0092). Patients who remained disease-free had higher levels of DOK-6 expression compared to those who died from breast cancer. CONCLUSION: Decreasing expression levels of DOK-2 and DOK- 6 with increased breast tumor progression supports the not that that DOK-2 and DOK-6 behave as tumor suppressors in human breast cancer.
其他文献
胃癌是世界上第三大肿瘤致死性疾病,其发病率在全球确诊的癌症中排行第五。胃癌产生原因纷繁复杂。除了幽门螺旋杆菌H. pylori感染,很多其他因素都能提高患病几率。引起胃癌转
“自由游戏活动”是幼儿主导的游戏活动,是幼儿最喜欢的活动.幼儿可自主选择游戏活动的空间、材料和同伴,自主发展游戏活动的情节和内容.自由游戏活动可以在室内,也可以在户
With the requirements of users enhanced for wireless communication,the cooperative communication will become a development trend in future.In this paper,a model
AIM: To determine the mutation status of human telomerase reverse transcriptase gene(TERT) promoter region in hepatocellular carcinoma(HCC) from different geogr
现阶段,古诗词教学已成为中小学语文教材中必不可少的内容之一.在新课标革新的背景下,其对低、高段古诗词教学存有差异化的要求,希望教师在古诗词整个授课过程中,需秉持着由
目的胚胎神经管畸形(Neural tube defects;NTD)是在胚胎早期发育过程中,由于胚胎神经管闭合不全引起的一类先天性发育缺陷,与多种环境因素的异常有关。通过动物实验已证实,高
中华优秀传统文化是中华民族在五千年的文化形成和发展过程中积淀孕育而成,是中华儿女的集体智慧的结晶,也是中华民族的宝贵财富.幼儿园开展优秀传统文化教育具有促进幼儿全
简介:王永保,江苏省东海职教中心校长,高级讲师。江苏省职业教育心理健康类专业教研中心组组长。江苏省首批职业教育领军人才校长班成员。  摘要:办学特色是中等职业学校可持续发展加速器和提升核心发展力的重要途径。在当今激烈的办学竞争中,办学特色的有无、高下直接关系到中等职业学校的实力和声誉,乃至于生存与发展。文章在对中等职业学校办学特色内涵进行界定的基础上,探讨了中等职业学校办学特色的内容并从校长思路、
我们读《论语》的时候,都会强烈地感受到其语言艺术的魅力.由此反推,不妨说,《论语》是一部文学书.至少可以说,它是一部具有浓厚文学气息的书.因为,文学是语言的艺术.本文从
游戏是一种以主动、自愿、愉悦为特征的“自由”活动,它是在幼儿已有经验的基础上展开的,符合每一个幼儿身心发展水平,有幼儿的地方就有游戏,游戏是幼儿生活、学习中不可分割