论文部分内容阅读
目的:探讨顺铂(cis-diamminedichlorop latinum DDP)和3,3-二吲哚基甲烷(3,3-diindolylmethane,DIM)联合应用诱导人卵巢癌细胞SK-OV-3凋亡的机制。方法:采用免疫组化技术观察细胞内caspase3蛋白的表达,利用RT-PCR和western blot检测bcl-2、caspase3和caspase9基因和蛋白表达变化。结果:细胞培养及免疫化学染色可见各给药组与对照组比较均有不同程度抑SK-OV-3细胞增殖并诱导其凋亡作用;RT-PCR结果显示各给药组均能使caspase3和caspase9基因表达上调,bcl-2表达下调;westernblot结果表明各给药组与对照组相比caspase3和caspase9蛋白表达上调,bcl-2表达下调,且0.4mg.L-1DDP+60μmol.L-1DIM联合应用与10倍剂量DDP(4mg/L-1)单独应用效果相同。结论:DIM与DDP均可抑制PC-3细胞增殖并诱导其凋亡;其细胞凋亡机制可能与上调caspase3、caspase9基因表达,下调bcl-2表达有关;DIM与DDP联合抗瘤具有明显的减毒增效作用。
AIM: To investigate the mechanism of cisplatin (cis-diamminedichloroplatinum DDP) and 3,3-diindolylmethane (DIM) combined with apoptosis inducing apoptosis in human ovarian cancer SK-OV-3 cells. Methods: The expression of caspase 3 protein was detected by immunohistochemistry. The expressions of bcl-2, caspase 3 and caspase 9 were detected by RT-PCR and western blot. Results: The results of cell culture and immunochemistry showed that the proliferation of SK-OV-3 cells and the induction of apoptosis of SK-OV-3 cells were significantly increased in all groups compared with the control group. The results of RT-PCR showed that the expressions of caspase3 and Westernblot results showed that the expression of caspase3 and caspase9 were up-regulated and the expression of bcl-2 was down-regulated compared with the control group, and the combination of 0.4mg.L-1DDP + 60μmol.L-1DIM Application Same effect as single dose of DDP (4mg / L-1) alone. Conclusions: Both DIM and DDP can inhibit the proliferation and induce the apoptosis of PC-3 cells. The mechanism of apoptosis may be related to the up-regulation of caspase3 and caspase9 gene expression and down-regulation of bcl-2 expression. Poison synergies.