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目的为探讨乳腺癌易感基因2(BRCA2)在喉鳞状细胞癌组织中的表达及临床意义。方法采用免疫组织化学染色法检测62例喉癌组织及23例声带息肉组织中BRCA2蛋白的表达并进一步分析其与临床病理指标的相关性,采用实时荧光定量PCR检测喉癌组织及对应的正常组织BRCA2 mRNA水平,Western blot法检测BRCA2蛋白水平。结果免疫组织化学染色结果显示BRCA2在喉癌中表达率为29.03%(18/62),在声带息肉中表达率为69.57%(16/23),与患者性别、年龄、吸烟、分化程度、颈淋巴结转移、临床分期无关,BRCA2表达水平与T分期呈负相关。与癌旁组织相比,喉鳞状细胞癌组织BRCA2mRNA和蛋白水平降低。结论 BRCA2在喉鳞状细胞癌组织表达降低,与T分期呈负相关。
Objective To investigate the expression of breast cancer susceptibility gene 2 (BRCA2) in laryngeal squamous cell carcinoma and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of BRCA2 protein in 62 cases of laryngeal carcinoma and 23 cases of vocal cord polyp. The correlation between the expression of BRCA2 protein and clinicopathological parameters was also analyzed. The real-time fluorescence quantitative PCR was used to detect the expression of BRCA2 protein in normal tissues BRCA2 mRNA levels, Western blot detection of BRCA2 protein levels. Results Immunohistochemical staining showed that the expression rate of BRCA2 in laryngeal carcinoma was 29.03% (18/62) and in vocal cord polyp, the expression rate was 69.57% (16/23), which was correlated with the gender, age, smoking, differentiation, Lymph node metastasis, clinical stage unrelated, BRCA2 expression level and T stage was negatively correlated. Compared with adjacent tissues, laryngeal squamous cell carcinoma BRCA2 mRNA and protein levels decreased. Conclusion The expression of BRCA2 is down-regulated in laryngeal squamous cell carcinoma and negatively correlated with T stage.