组织块法原代培养人牙周膜细胞,经来源鉴定后,取生长良好的第4代细胞用LPS进行刺激,分别于刺激0、4、8、12、24h,运用免疫细胞化学染色法检测TLR2和TLR4在牙周膜细胞中的表达;利用多功能真彩色细胞分析管理系统进行图像分析,对TLR2和TLR4进行免疫组化评分(IHS)。结果:无LPS刺激的对照组(刺激Oh)TLR2和TLR4的表达均为弱阳性,加入LPS后,染色增强,且随着刺激时间的延长而 Tissue culture of human primary cultured human periodontal ligament cells, identified by the source, the well-growing fourth generation of cells were stimulated with LPS, were stimulated 0,4,8,12,24 h, using immunocytochemical staining Expression of TLR2 and TLR4 in periodontal ligament cells. Immunohistochemical scores (IHS) of TLR2 and TLR4 were assessed using a versatile true colorimetric analysis system. Results: The expression of TLR2 and TLR4 in LPS-stimulated control group (stimulated Oh) were both weakly positive. The staining was enhanced after addition of LPS, and with the prolongation of stimulation time