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Objective: To evaluate the effect of HIF-1 αover-expression on angiogenesis in human prostate cancer cells. Methods:LNCaP cells(a human prostate cancer cell line) were transfected with the recombinant plasmid pcDNA3.1( -)-HIF-1αwith Lipo-fectamine 2000 system. The positive clones were selected by G418 being further confirmed by Western blot and immunofluores-cence. The expression levels of VEGF, iNOS and Ang-Ⅱ were determined. Results: The expression of HIF-1αin the LNCaP/HIF1αcells was significantly increased in transfected cells, which induced the up-regulation of VEGF, iNOS, whereas Ang-Ⅱexpression remained un- changed. Conclusion: Over-expression of HIF-1αcan induce angiogenesis proteins and may improve theangiogenesis potency of prostate cancer.
Objective: To evaluate the effect of HIF-1αover-expression on angiogenesis in human prostate cancer cells. Methods: LNCaP cells (a human prostate cancer cell line) were transfected with the recombinant plasmid pcDNA3.1 (-) - HIF- -fectamine 2000 system. The positive clones were selected by G418 still further confirmed by Western blot and immunofluorescence-cence. The expression levels of VEGF, iNOS and Ang-II were determined. Results: The expression of HIF-1αin the LNCaP / HIF1α cells was Over-expression of HIF-1αcan induce angiogenesis proteins and may improve the angiogenesis potency of prostate cancer.