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目的探讨基底细胞样乳腺癌(BLBC)和非基底细胞样乳腺癌(NBLBC)癌组织中TopoⅡα、C-MYC基因扩增与Ki-67表达的关系。方法选取2010年2月~2014年8月在该院临床病理诊断中心经石蜡包埋的BLBC及NBLBC癌组织各100例,同时选取100例周围正常的乳腺组织为对照。结果 TopoⅡα与C-MYC基因改变类型主要为扩增与缺失,对照组无TopoⅡα、C-MYC和Ki-67表达。100例BLBC病理组织中,TopoⅡα与C-MYC基因扩增分别为43例和51例,缺失分别为57例49例;Ki-67阴性24例、阳性76例;Spearman等级相关分析显示,TopoⅡα、C-MYC基因扩增与Ki-67表达相关(P<0.05)。100例NBLBC病理组织中,TopoⅡα与C-MYC基因扩增分别为扩增34例和43例,缺失66例和57例;Ki-67阴性16例、阳性84例;Spearman等级相关分析显示,TopoⅡα、C-MYC基因扩增与Ki-67表达相关(P<0.05),TopoⅡα与C-MYC基因扩增呈正相关关系(P<0.05)。与NBLBC比,BLBC的TopoⅡα与C-MYC基因扩增略多。结论 TopoⅡα、CMYC基因扩增与Ki-67相关,BLBC癌组织的TopoⅡα、C-MYC基因扩增多于NBLBC癌组织,TopoⅡα与C-MYC基因扩增可能参与乳腺癌的发生发展过程。
Objective To investigate the relationship between TopoⅡα and C-MYC gene amplification and Ki-67 expression in basal-like breast cancer (BLBC) and non-basal cell carcinoma (NBLBC). Methods From February 2010 to August 2014, 100 cases of BLBC and NBLBC in paraffin-embedded clinical and pathological diagnosis center of the hospital were selected, and 100 cases of normal breast tissue were selected as control. Results The types of TopoⅡα and C-MYC genes were mainly amplified and deleted. The control group showed no expression of TopoⅡα, C-MYC and Ki-67. In 100 cases of BLBC pathology, TopoⅡα and C-MYC gene amplification were 43 cases and 51 cases, the deletion was 57 cases in 49 cases; Ki-67 negative in 24 cases, positive in 76 cases; Spearman rank correlation analysis showed Topo Ⅱ α, C-MYC gene amplification correlated with Ki-67 expression (P <0.05). In 100 cases of NBLBC pathology, TopoⅡα and C-MYC gene amplification were amplified in 34 cases and 43 cases, 66 cases were missing and 57 cases; Ki-67 negative in 16 cases, 84 cases were positive; Spearman rank correlation analysis showed Topo Ⅱ α , C-MYC gene amplification and Ki-67 expression (P <0.05), TopoⅡα and C-MYC gene amplification was positively correlated (P <0.05). Compared with NBLBC, BLBC's TopoⅡα and C-MYC gene amplification slightly more. Conclusion The amplification of TopoⅡα and CMYC genes is correlated with Ki-67. The expression of TopoⅡα and C-MYC in BLBC is more than that in NBLBC. The amplification of TopoⅡα and C-MYC may be involved in the development of breast cancer.