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以河北杨(Populus hopeiensis)为材料,研究拟南芥(Arabidopsis thaliana)油菜素内酯(BR)生物合成酶基因DAS5对其生长表型、生物量及抗旱性的影响。结果表明:(1)转DAS5基因河北杨植株的根长、地径、叶柄及叶片长度均显著大于野生型植株,且地上、地下部分干重及根冠比显著高于野生型,其拥有发达的根系;(2)在干旱胁迫下,转DAS5基因河北杨植株失水褪绿速度较野生型植株缓慢,在复水后转基因植株能够较早较好地恢复活力,萌发较多的新幼芽且长势良好;(3)控水期间,转基因河北杨的相对生长率显著高于野生型,且随着干旱胁迫程度的加剧,其可溶性糖含量、游离脯氨酸含量、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性均显著高于野生型。实验结果表明,与野生型相比,转基因植株具有较高的生长量与较强的抗干旱胁迫能力,说明来自拟南芥的BR生物合成酶基因DAS5可以显著增加河北杨的生长量并在抵御干旱胁迫机制中发挥重要作用。
Populus hopeiensis was used to study the effects of DAS5 on the growth phenotype, biomass and drought resistance of Arabidopsis thaliana brassinosteroid (BR) biosynthesis genes. The results showed that: (1) The root length, diameter, petiole and leaf length of transgenic DAS5 transgenic Hebei were significantly higher than those of wild type, and the dry weight and root / shoot ratio aboveground and underground were significantly higher than that of wild type. Under the drought stress, the transplanting plants of Hebei DAS5 transgenic plants with DAS5 gene were dehydrated more slowly than the wild-type plants. After rewatering, the transgenic plants were able to recover their vigor earlier and germinated more new shoots (3) During the water control period, the relative growth rate of transgenic Hebei was significantly higher than that of wild type. With the aggravation of drought stress, the soluble sugar content, free proline content, catalase (CAT) ) Activity, superoxide dismutase (SOD) activity were significantly higher than the wild type. The results showed that compared with the wild-type, the transgenic plants had higher growth capacity and stronger resistance to drought stress, indicating that the BR biosynthetic enzyme gene DAS5 from Arabidopsis thaliana could significantly increase the growth of Hebei poplar, Drought stress mechanisms play an important role.