论文部分内容阅读
由爱德华氏细菌引起的肠败血症(ESC,又称爱德华氏病)是影响斑点叉尾养殖最大的疾病。为快速和准确的诊断该疾病,本研究以NCBI公布的爱德华氏细菌eip基因(GeneBank登录号为AF037441)为模板序列,设计种特异性诊断引物,成功建立了用于斑点叉尾肠败血症病原菌的PCR检测方法。研究结果表明,该方法能够直接从发病斑点叉尾脑、肝、脾和肾组织中检测到爱德华氏菌,检测的最低量为21个细菌;同时,该诊断方法与I型荧光假单胞菌、点状产气单胞菌点状亚种、鳗弧菌、温和气单胞菌、肠型点状产气单胞菌、柱状黄杆菌、嗜水气单胞菌、河弧菌、豚鼠气单胞菌及海豚链球菌等常见水产病原菌无交叉反应。临床样品检测证明,本研究所建立的PCR检测方法可以用于斑点叉尾肠败血症的快速诊断。
Enteric septicemia (ESC, also known as Edwards’ s disease), caused by Edwardsiella bacteria, is the most affected disease in Channel Catfish cultivation. In order to rapidly and accurately diagnose the disease, the eukaryotic bacteria eip gene (GeneBank accession number AF037441) published by the NCBI was used as a template sequence to design species-specific diagnostic primers. Pathogen PCR detection method. The results showed that this method could detect E. bovis directly from the brain of the catfish, liver, spleen and kidney, and the lowest detection limit was 21 bacteria. Meanwhile, Bacillus subtilis, Pseudomonas aeruginosa spot-like subspecies, Vibrio anguillarum, Aeromonas sobria, Aeromonas punctatus, Flavobacterium levum, Aeromonas hydrophila, Guinea pig Aeromonas and Streptococcus dyscalin and other common aquatic pathogens no cross-reaction. Clinical sample testing proved that the PCR test method established in this study can be used for the rapid diagnosis of catfish intestinal sepsis.