ENZYMOLOGY相关论文
Characterization of the First Fungal Glycosyl Hydrolase Family 19 Chitinase(NbchiA)from Nosema bomby
Chitinases(EC 3.2.1.14),as one kind of glycosyl hydrolase,hydrolyze the β-(1,4)linkages of chitin.According to the sequ......
Enzymes are the major players for many biological processes. Fundamental studies of the enzymatic activity at the single......
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NADH-glutamate dehydrogenase (GDH) is active in human tissues, and is chromatographically purified, and studied because ......
The catalytic efficiencies (kcat/Km),reflecting the relative rates of an enzyme acting on different substrates in co......
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,The Presence and Localization of Thioredoxins in Diatoms,Unicellular Algae of Secondary Endosymbiot
Diatoms are unicellular algae of great ecological importance.So far,very little is known about the regulation of carbon ......
A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been character-ized and seve......
,Prompt and Easy Activation by Specific Thioredoxins of Calvin Cycle Enzymes of Arabidopsis thaliana
The Calvin cycle enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK) can form under o......
,Sensing Sulfur Conditions: Simple to Complex Protein Regulatory Mechanisms in Plant Thiol Metabolis
Sulfur is essential for plant growth and development, and the molecular systems for maintaining sulfur and thiol metabol......
Background It was reported that telomerase expression is closely associated with cellular immortality and cancer. This s......
为解决酶学难以作为一门独立课程的形式纳入本科教学体系的困境,将酶学核心知识整合为理论课堂教学模块、实验教学模块、模拟仿真......
AspergilusficuumCN92植酸酶是经NTG和Co60γ射线复合诱变发酵产生的菌株,用冷乙醇沉淀析酶而制得的植酸酶粗酶粉为实验样品,植酶钠(P3168,Sigma公司产)为底物,考察CN92植酸酶......
目的研究腹腔镜胆囊切除术肝脏病理与酶学变化的原因.方法 1999~2001年我院收治的69例胆囊结石随机分组.常规应用单极高频电刀行腹......
以红曲霉、米曲霉为菌种,以豆粕、米、麸皮等为原料,经微生物培养工艺制得成曲,并对此进行了酶学研究。在此基础上确定了曲料配比等工......
目的观察双酚A对小鼠生殖内分泌系统的影响。方法小鼠经不同剂量的双酚A染毒3d后,用紫外分光光度法检测睾丸组织中乳酸脱氢酶(LDH)、......
目的:该研究探讨维甲酸对Bel-7402人肝癌细胞的分化诱导作用,为肝癌的分化治疗提供资料。方法:用维甲酸处理Bel-7402人肝癌细胞,观察细胞增殖、软琼脂集......
传统的物理化学方法在去除重金属方面存在二次污染和投资成本大等不足。而生物固定化是一种可有效处理含金属废水的技术,在处理含重......
饲料组合酶是专门针对酶制催化的高效问题而提出,它与一般的复合酶不同。应用饲料组合酶可以降低饲料酶制剂的使用成本,对提高非常规......
目的通过在20家获批国家临床重点专科的检验科间开展酶学指标的实验室间比对,探讨临床化学酶学检测的实验室间结果互认的可行性与可......
目的为了解烧伤早期影响肝功能的相关因素.方法对某省级医院1997-2001年收治的497例病人的丙氨酸转氨酶(ALT),部分病人的天冬氨酸......
目的:探讨10q部分单体综合征患者生理病变化及其诊断。方法:全血微量法培养外周血淋巴细胞、染色体行G、C式显带。分别用速率法、分光光度......
空间排斥色谱法是一种高分子聚合物的柱分级分离技术,通过这种方法可以使柱中溶解的聚合物分子按其大小被分离。空间排斥色谱法在生......
目的应用Agilent系统,参照国际临床化学联合会所提供的参考方法对血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、乳酸脱氢......
绿色木霉926液体达发酵终点(6~7d)后,所得纤维素酶中CMC酶活最高可达20U/ml,滤纸酶活最高可达5.5U/ml.发酵液纤维素酶中CMC酶组分经硫酸铵沉淀,SephadexG—100柱层析后可提纯6倍左......
采用比色法和电泳技术对引起成年国槐根茎腐烂病的5种镰刀菌的酶学特性进行了分析,为镰刀菌的分类和生理分化研究等提供理论依据。......
目的: 对107例中枢神经系统疾病患者脑脊液(CSF)CPK,LDH,AST,α-HBD和ALT活性进行测定,探讨各疾病酶活性变化. 方法:被检测CSF,离......
概述了抗有机农药的不同昆虫的酶类的活性变化。抗性品系的水解酶类和氧化酶类均有明显的变化,即对水解酶类而言,酯酶活力尤其是羟酸......
Maximum lignocellulose yield of biomass sorghum [Sorghum bicolor L. (Moench.)] is hampered by complex biological phenome......
从宇佐美曲霉(A.usamii) 固态发酵物中提取纤维素酶.粗酶液经硫酸铵盐析除去大量杂蛋白,然后通过2次Sephadex G-200 柱层析纤维素......
目的探讨环氧化酶-2(COX-2)在肝细胞癌发生发展中的作用及其意义.方法用免疫组织化学的方法检测41例肝细胞癌组织、16例乙型肝炎后......
Lipase from Antarctic krill,with a molecular weight of 71.27kDa,was purified with ammonium sulfate precipitation and a s......
膜泡是细菌外膜向外膨出呈芽状,在形成独立成份后游离进入周围微环境的一种泡状膜结构,是许多G~-菌的一种适应性或功能性生物学特征,......
目的: 模拟人的患病因素制作胃癌前病变大鼠模型,观察加味左金丸对胃癌前病变的治疗作用并从分子水平探讨其作用机理。 方法......
It has been a dream that theoretical biology can be extensively applied in experimental biology to accelerate the unders......