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目的建立兔颈脊髓半侧挫伤模型,观察不同程度损伤24小时后其MRI及组织学表现。方法 22只成年雄性新西兰兔,随机分为中度损伤组(n=9)、重度损伤组(n=9)和假手术组(n=4)。直径为3.0 mm的打击头由电磁伺服材料试验机驱动,对准C_5脊髓左侧行高速挫伤(500 mm/s)。根据打击头的位移距离分为位移2.0 mm组(中度损伤组)和位移2.8 mm组(重度损伤组)。假手术组仅暴露C_5脊髓,不进行挫伤。损伤后24小时每组随机取两例行MRI影像学检查,所有动物均取材进行组织学观察,测量横断面脊髓出血面积。结果中度损伤组打击力和位移分别为(2.47±0.39)N和(1.99±0.02)mm,重度损伤组打击力和位移分别为(5.16±0.82)N和(2.76±0.02)mm,中度损伤组的打击力明显小于重度损伤组(P<0.05)。MRI结果显示,中度及重度损伤组均可见C_5左侧脊髓信号改变。HE染色显示脊髓左侧有明显的出血及脊髓组织结构破坏,中度损伤组损伤中心横截面出血面积(0.012±0.006)mm~2明显小于重度损伤组(0.039±0.006)mm~2(P<0.05)。结论本文建立的兔颈脊髓半侧挫伤模型能够控制挫伤位移,实现对脊髓的高速打击。不同程度的颈脊髓半侧挫伤在打击力、MRI影像学及组织学上均有不同。
Objective To establish a hemisection model of cervical spinal cord in rabbits and observe the MRI and histological findings after 24 hours of injury. Methods Twenty-two male New Zealand white rabbits were randomly divided into moderate injury group (n = 9), severe injury group (n = 9) and sham operation group (n = 4). The striking head with a diameter of 3.0 mm was driven by an electromagnetic servo material testing machine and aligned with a high speed contusion (500 mm / s) on the left side of the C_5 spinal cord. According to the displacement of the striking head, it is divided into 2.0 mm displacement group (moderate injury group) and 2.8 mm displacement group (severe injury group). Sham-operation group only exposed C_5 spinal cord, without contusion. Twenty-four hours after injury, each group was randomly selected two cases of MRI imaging examination, all animals were drawn for histological observation, measuring the cross-sectional area of spinal cord hemorrhage. Results The strike force and displacement of moderate injury group were (2.47 ± 0.39) N and (1.99 ± 0.02) mm, respectively. The strike force and displacement of severe injury group were (5.16 ± 0.82) N and (2.76 ± 0.02) mm, respectively The impact of injury group was significantly less than severe injury group (P <0.05). MRI results showed that in the moderate and severe injury group can be seen on the C_5 left spinal cord signal changes. Hematoxylin and eosin (HE) staining showed obvious hemorrhage and structural damage of spinal cord on the left side of the spinal cord. The mean cross-sectional area of hemorrhage (0.012 ± 0.006) mm ~ 2 in moderate injury group was significantly lower than that in severe injury group (0.039 ± 0.006) 0.05). Conclusion The model of hemisection caused by cervical spinal cord in this paper can control the contusion displacement and achieve high-speed spinal cord injury. Different degrees of half cervical spinal cord contusion in striking power, MRI imaging and histology are different.